HY5 antibody, rabbit polyclonal

Background

Transcription factor HY5 is a nuclear protein that belongs to the bZIP family, which promotes photo

morphogenesis in light. HY5 acts downstream of the light receptor network and directly affects transcription of

light-induced genes. HY5 is specifically involved in the blue light specific pathway, suggesting that it participates

in transmission of cryptochromes (CRY1 and CRY2) signals to downstream responses. In darkness, its degradation

prevents the activation of light-induced genes. Negatively regulated by Cop1. Although cytokinins do not appear to

affect the gene's promoter activity, they appear to stabilize the protein. HY5 plays a role in anthocyanin

accumulation in far-red light and blue light, but not in red light or in the dark. Mutant studies showed that the gene

product is involved in the positive regulation of the PHYA-mediated inhibition of hypocotyl elongation. Binds to

G- and Z-boxes, and other ACEs, but not to E-box. Loss of function mutation shows ABA resistant seedling

phenotypes suggesting involvement for HY5 in mediating ABA responses. Binds to the promoter of ABI5 and

regulates its expression.

Other names: ELONGATED HYPOCOTYL 5, HY5, REVERSAL OF THE DET PHENOTYPE 5, TED 5

Immunogen: Recombinant protein

Concentration: 1.0mg/ml.

Expected/apparent MW: 18 /36 kDa

Species specificity: Arabidopsis thaliana

Tested applications: Western blot (WB), IF

Recommended dilution: 1 : 500-2000 (WB, detect endogenous protein ), 1: 100 - 1: 500 (IF)(optimal dilutions

should be determined by the end use.)

Storage Buffer: PBS Buffer, pH7.4

Storage: Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Stable for 2

years from date of receipt. Aliquot to avoid repeated freezing and thawing