RPT5 antibody, rabbit polyclonal

Background

Encodes RPT5a (Regulatory Particle 5a), one of the six AAA-ATPases of the proteasome regulatory particle.

Essential for gametophyte development. In Arabidopsis, the RPT5 subunit is encoded by two highly homologous

genes, RPT5a and RPT5b. RPT5a and RPT5b show accession-dependent functional redundancy. In Wassilewskija

(Ws) accession: mutant alleles of RPT5a displayed 50% pollen lethality, indicating that RPT5a is essential for

male gametophyte development. In the Columbia (Col) accession, a rpt5a mutant allele did not display such a

phenotype because the RPT5b Col allele complements the rpt5a defect in the male gametophyte, whereas the

RPT5b Ws allele does not. Double rpt5a rpt5b mutants in Col background showed a complete male and female

gametophyte lethal phenotype. The mRNA is cell-to-cell mobile.

Other names: ATS6A.2, REGULATORY PARTICLE TRIPLE-A ATPASE 5A, RPT5A.

Immunogen: Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein

containing the C-terminal region of Arabidopsis thaliana RPT5A (AT3G05530). Antibodies were purified by

affinity purification using immunogen.

Concentration: 1.0mg/ml.

Expected/apparent MW: 47 kDa

Species specificity: Arabidopsis thaliana

Tested applications: Western blot (WB), IF

Recommended dilution: 1 : 500-2000 (WB, detect endogenous protein ), 1: 100 - 1: 500 (IF)(optimal dilutions

should be determined by the end use.)

Storage Buffer: PBS Buffer, pH7.4

Storage: Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Stable for 2

years from date of receipt. Aliquot to avoid repeated freezing and thawing.

* Please note: FOR RESEARCH USE ONLY