DCL1 antibody, rabbit polyclonal

Background

Encodes a Dicer homolog. Dicer is a RNA helicase involved in microRNA processing. Mutations in this locus can

result in embryo lethality. Embryo shape at seed maturity is globular-elongate. Other mutants convert the floral

meristems to an indeterminate state, others yet show defects in ovule development. mRNA is expressed in all shoot

tissues. DCL1 is able to produce miRNAs and siRNAs. The mRNA is cell-to-cell mobile.

Other names: ABNORMAL SUSPENSOR 1, ASU1, ATDCL1, CAF, CARPEL FACTORY, DCL1, DICER-LIKE

1, EMB60, EMB76, EMBRYO DEFECTIVE 60, EMBRYO DEFECTIVE 76, SHORT INTEGUMENTS 1, SIN1,

SUS1, SUSPENSOR 1

Immunogen: KLH-conugated synthetic peptide, chosen from Arabidopsis thaliana DCL1 protein sequence

AT1G01040.

Concentration: 1.0mg/ml

Expected/apparent MW: 213 kDa

Tested applications: Western blot (WB), IF

Species specificity: Arabidopsis thaliana

Recommended dilution: 1 : 500-2000 (WB, detect endogenous protein ), 1: 100 - 1: 500 (IF)(optimal dilutions

should be determined by the end use.)

Storage Buffer: PBS Buffer, pH7.4

Storage: Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Stable for 2

years from date of receipt. Aliquot to avoid repeated freezing and thawing.

* Please note: FOR RESEARCH USE ONLY